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  • Periodicals (Báo – Tạp chí)

  • A clinically-relevant polymorphism in the Na+/taurocholate cotransporting polypeptide (NTCP) occurs at a rheostat position

    • Authors : Strzelecka, Dominika; Smietanski, Miroslaw (2020)

  • Chemical modifications enable preparation of mRNAs with augmented stability and translational activity. In this study, we explored how chemical modifications of 5’,3’-phosphodiester bonds in the mRNA body and polyA tail influence the biological properties of eukaryotic mRNA. To obtain modified and unmodified in vitro transcribed mRNAs, we used ATP and ATP analogues modified at the α-phosphate (containing either O-to-S or O-to-BH3 substitutions) and three different ...
  • Periodicals (Báo – Tạp chí)

  • Public LC-Orbitrap-MS/MS Spectral Library for Metabolite Identification

    • Authors : Phapale, Prasad (2020)

  • Liquid chromatography-mass spectrometry(LC-MS)-based untargeted metabolomics studies require high-quality pectral libraries for reliable metabolite identification. We have constructed EMBL-MCF, an open LC-MS/MS pectral library that currently contains over 1600 fragmentation spectra from 435 authentic standards of dogenous metabolites and lipids. The unique features of the library are presence of chromatographic profiles cquired with different LC-MS methods and coverage of different adduct...
  • Journal article

  • An untargeted metabolomics strategy to measure differences in metabolite uptake and excretion by mammalian cell lines

    • Authors : Muelas, Marina Wright; Roberts, Ivayla; Mughal, Farah (2020)

  • Introduction It is widely but erroneously believed that drugs get into cells by passing through the phospholipid bilayer portion of the plasma and other membranes. Much evidence shows, however, that this is not the case, and that drugs cross biomembranes by hitchhiking on transporters for other natural molecules to which these drugs are structurally similar. Untargeted metabolomics can provide a method for determining the differential uptake of such ...
  • Journal article

  • Standard Flow Multiplexed Proteomics (SFloMPro) – An Accessible and Cost-Effective Alternative to NanoLC Workflows

    • Authors : Jenkins, Conor; Orsburn, Ben (2020)

  • Describing a method for performing multiplexed proteomics without the use of NanoLC by using offline fractionation of labeled peptides followed by rapid “standard flow” HPLC gradient LC-MS/MS. Standard Flow Multiplexed Proteomics (SFloMPro) enables high coverage quantitative proteomics of up to 16 mammalian samples in about 24 hours. In this study, we compare NanoLC and SFloMPro analysis of fractionated samples. Our results demonstrate that comparable data is...
  • Journal article

  • Mass spectrometric characterisation of the circulating peptidome following oral glucose ingestion in control and gastrectomised patients

    • Authors : Kay, Richard G; Foreman, Rachel E; Roberts, Geoff P (2020)

  • Meal ingestion triggers secretion of a variety of gut and endocrine peptides, several of which are routinely measured in research studies by commercial immunoassays. We developed an LC-MS/MS based assay for parallel monitoring of multiple peptides in small volumes of human plasma, providing the benefit of analysing exact peptide sequences rather than immuno-reactivity, and potential advantages of cost and sample volumes for measuring multiple peptide hormones...