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  • Periodicals (Báo – Tạp chí)

  • Amphipathic Fluorescent Dyes for Sensitive and Long-Term Monitoring of Plasma Membranes

    • Authors : Daly, Leonard (2020)

  • Adaption of cells to low oxygen environments is an essential process mediated in part by the Hypoxia Inducible Factors (HIFs). Like other transcription factors, the stability and transcriptional activity of HIFs, and consequently he hypoxic response, are regulated by post-translational modification (PTM) and changes in biomolecular interactions. However, our current understanding of PTM-mediated regulation of HIFs is primarily based on in vitro protein fragment-based s...
  • Journal article

  • Use of the Polo-like kinase 4 (PLK4) inhibitor centrinone to investigate intracellular signaling networks using SILAC-based phosphoproteomics

    • Authors : Byrne, Dominic P; Clarke, Christopher J; Brownridge, Philip J (2020)

  • Polo-like kinase 4 (PLK4) is the master regulator of centriole duplication in metazoan organisms. Catalytic activity and protein turnover of PLK4 are tightly coupled in human cells, since changes in PLK4 concentration and catalysis have profound effects on centriole duplication and supernumerary centrosomes, which are associated with aneuploidy and cancer. Recently, PLK4 has been targeted with a variety of small molecule kinase inhibitors exemplified by centrinone...
  • Journal article

  • Actin filament oxidation by MICAL1 suppresses protections from cofilin-induced disassembly

    • Authors : Wioland, Hugo; Frémont, Stéphane; Guichard, Bérengère (2020)

  • Proteins of the ADF/cofilin family play a central role in the disassembly of actin filaments, and their activity must be tightly regulated in cells. Recently, the oxidation of actin filaments by the enzyme MICAL1 was found to amplify the severing action of cofilin through unclear mechanisms. Two essential factors normally prevent filament disassembly: the inactivation of cofilin by phosphorylation, and the protection of filaments by tropomyosins, but...
  • Journal article

  • The relationship between effective molarity and affinity governs rate enhancements in tethered kinase-substrate reactions

    • Authors : Speltz, Elizabeth B; Zalatan, Jesse G (2020)

  • Scaffold proteins are thought to accelerate protein phosphorylation reactions by tethering kinases and substrates together, but there is little quantitative data on their functional effects. To assess the contribution of tethering to kinase reactivity, we compared intramolecular and intermolecular kinase reactions in a minimal model system. We find that tethering can enhance reaction rates in a flexible tethered kinase system, and the magnitude of the effect ...
  • Journal article

  • The cryo-electron microscopy structure of the human CDK-activating kinase

    • Authors : Grebera, Basil J; Bertoldic, Juan M. Perez; Limd, Kif (2020)

  • Control of gene expression and the cell cycle is critical for appropriate cell growth and timely cell division. Failure of the mechanisms regulating these processes can result in proliferative diseases. A molecular complex termed the CDK activating kinase (CAK) impinges on both of these regulatory networks in human cells and is thus a possible drug target for treatment of cancer. Here, we use cryo-electron microscopy to describe the detailed mo...