- Journal article
Authors : Koch, Amanda; Aguilera, Luis; Morisaki, Tatsuya (2020) - Viruses use IRES sequences within their RNA to hijack translation machinery and thereby rapidly replicate in host cells. While this process has been extensively studied in bulk assays, the dynamics of hijacking at the single-molecule level remain unexplored in living cells. To achieve this, we developed a bicistronic biosensor encoding complementary repeat epitopes in two ORFs, one translated in a Cap-dependent manner and the other translated in an ...
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- Journal article
Authors : Drino, Aleksej; Oberbauer, Vera; Troger, Conor (2020) - During particular stress conditions, transfer RNAs (tRNAs) become substrates of stress-induced endonucleases, resulting in the production of distinct tRNA-derived small RNAs (tsRNAs). These small RNAs have been implicated in a wide range of biological processes, but how isoacceptor and even isodecoder-specific tsRNAs act at the molecular level is still poorly understood. Importantly, stress-induced tRNA cleavage affects only a few tRNAs of a given isoacceptor or i...
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- Journal article
Authors : Hendler, Adi; Akiva, Eyal; Sandhu, Mahakaran (2020) - Many enzymes that catalyze protein post-translational modifications (PTM) can specifically modify multiple target proteins. However, little is known regarding the molecular basis and evolution of multi-specificity in these enzymes. Here, we used bioinformatics and experimental approaches to investigate the molecular basis and evolution of multi-specificity in the sirtuin-1 (SIRT1) deacetylase. Guided by bioinformatics analysis of SIRT1 orthologues and substrates, we identified and&#x...
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- Journal article
Authors : Mrozowich, Tyler; Henrickson, Amy; Demeler, Borries (2020) - Viral infections are responsible for numerous deaths worldwide. Flaviviruses, which contain RNA as their genetic material, are one of the most pathogenic families of viruses. There is an increasing amount of evidence suggesting that their 5’ and 3’ non-coding terminal regions are critical for their survival. In this study, the 5’ and 3’ terminal regions of Murray Valley Encephalitis and Powassan virus were examined using biophysical and computational...
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- Journal article
Authors : Sun, Kuanxiang; Tian, Wanli; Liu, Wenjing (2020) - PS flippase (P4-ATPase) transports PS from the outer to the inner leaflet of the lipid bilayer in the membrane to maintain PS asymmetry, which is important for biological activity of the cell. ATP11A is expressed in multiple tissues and plays a role in myotube formation. However, detailed cellular function of ATP11A remains elusive. Mutation analysis revealed that I91, L308 and E897 residues in ATP8A2 are important for flippase activity. In...
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- Journal article
Authors : Hubert, Madlen; Larsson, Elin; Vegesna, Naga Venkata Gayathri (2020) - Caveolae serve as mechanoprotectors and membrane buffers but their specific role in sensing plasma membrane lipid composition remains unclear. Hubert et al. show that cholesterol and glycosphingolipids accumulate in caveolae and drive subsequent scission from the cell surface. These results provide new insight into how lipids contribute to budding and scission of membrane domains in cells.
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- Journal article
Authors : Ngo, Tony; Stephens, Bryan S; Gustavsson, Martin (2020) - Reporting the discovery of the geometry of the complex between full-length CXCR4, a prototypical CXC receptor and driver of cancer metastasis, and its endogenous ligand CXCL12. By comprehensive disulfide crosslinking, we establish the existence and the structure of a novel interface between the CXCR4 distal N-terminus and CXCL12 β1-strand, while also recapitulating earlier findings from NMR, modeling and crystallography of homologous receptors. A crosslinking-informed ...
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- Journal article
Authors : Barra, Angélica Luana C; Dantas, Lívia de Oliveira C; Morão, Luana Galvão (2020) - The antibiotic resistance is a worldwide concern that requires a concerted action from physicians, patients, governmental agencies and academia to prevent infections and the spread of resistance, to track resistant bacteria, to improve the use of current antibiotics and to develop new antibiotics. Despite the efforts spent so far, the current antibiotics in the market are restricted to only five general targets/pathways highlighting the need for basic&#x...
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- Journal article
Authors : Qian, Chen; Niu, Ben; Jimenez, Rod Brian (2020) - Describing the development of a fully automated peptide mapping procedure with a high-throughput robotic liquid handling system to improve sample handling capability and outcome reproducibility while saving analyst hands-on time. The automated procedure is completely hands-free, and setup requires the analyst only to prenormalize the sample concentrations and load buffers and reagents at their designated positions on the robotic deck. The robotic liquid handler perform...
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- Journal article
Authors : Gu, Lei; Wang, Longfei; Chen, Hao (2020) - Recent studies reported that METTL4 regulates DNA 6mA in vivo and therefore is a candidate DNA m6A methyltransfease. However, the enzymatic activity of METTL4 in vitro has not been demonstrated in part due to the difficulties of obtaining well-folded proteins. Here we show that mettl4 is a major methyltransfase responsible for m6A methylation of U2 snRNA both in vitro and in vivo in fly, and identify adenosine at 29th position as the&...
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