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  • Periodicals (Báo – Tạp chí)

  • Extension of a de novo TIM barrel with a rationally designed secondary structure element

    • Authors : Wiese, Jonas Gregor (2020)

  • To explore opportunities to extend and diversify this scaffold by adding a short de novo helix on top of the barrel. Due to the size of the protein we developed a design pipeline based on computational ab initio folding that solves a less complex sub-problem focused around the helix and its vicinity and adapt it to the entire protein. We provide biochemical characterization and a high-resolution X-ray structure for one variant and...
  • Journal article

  • Hsf1 is SUMOylated in the activated trimeric state

    • Authors : Kmiecik, Szymon W.; Drzewicka, Katarzyna; Melchior, Frauke (2020)

  • According to cell biological and proteomics data, Hsf1 is also modified by SUMO (small ubiquitin-like modifier) at several sites. How SUMOylation affects Hsf1 activity at a molecular level is still unclear. Here, we analyzed Hsf1 SUMOylation in vitro with purified components to address questions that could not be answered in cell culture models. In vitro Hsf1 is primarily conjugated at lysine 298 with a single SUMO, though we did detect lo...
  • Journal article

  • Cytoplasmic short linear motifs in ACE2 and integrin b3 link SARS-CoV-2 host cell receptors to endocytosis and autophagy

    • Authors : Kliche, Johanna; Ali, Muhammad; Ivarsson, Ylva (2020)

  • The spike protein of the SARS-CoV-2 interacts with angiotensin converting enzyme 2 (ACE2) and enters the host cell by receptor-mediated endocytosis. Concomitantly, evidence is pointing to the involvement of additional host cell receptors, such as integrins. The cytoplasmic tails of ACE2 and integrin β3 contain a plethora of predicted binding motifs. Here, we confirm the functionality of some of these motifs through affinity measurements. The class I PDZ&...
  • Ebooks (Sách điện tử)

  • Disentangling the contribution of each descriptive characteristic of every single mutation to its functional effects

    • Authors : Sruthi, C. K.; Prakash, Meher K. (2020)

  • To address the former aspect using a framework of interpretable AI. Relations between the physico-chemical descriptors and their contributions to the mutational effects are extracted by analyzing the data on 29,832 variants from 8 systematic deep-mutational scan studies. It is found that the intuitive dependences of fitness and solubility on the distance of the amino acid from active site could be extracted and quantified. The dependence of the muta...