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  • Journal article

  • Dynamics of RNA polymerase II and elongation factor Spt4/5 recruitment during activator-dependent transcription

    • Authors : Rosen, Grace A; Baek, Inwha; Friedman, Larry J (2020)

  • The synthesis of a eukaryotic messenger RNA molecule involves the association of RNA polymerase and dozens of accessory proteins on DNA. We used differently colored fluorescent dyes to tag DNA, RNA polymerase II, and the elongation factor Spt4/5 in yeast nuclear extract, and then observed the assembly and dynamics of individual molecules of the proteins with single DNA molecules by microscopy. The observations quantitatively define an overall pathwa...
  • Journal article

  • Rdh54/Tid1 Inhibits Rad51-Rad54-Mediated D-loop Formation and Limits D-loop Length

    • Authors : Shah, Shanaya Shital; Hartono, Stella; Piazza, Aurèle (2020)

  • Displacement loops (D-loops) are intermediates formed during homologous recombination that play a pivotal role in the fidelity of repair. Rdh54 (a.k.a. Tid1), a Rad54 paralog in Saccharomyces cerevisiae, is well-known for its role with Dmc1 recombinase during meiotic recombination. Yet contrary to Dmc1, Rdh54 is also present in somatic cells where its function is less understood. While Rdh54 enhances the Rad51 DNA strand invasion activity in vitro, it&#x...
  • Journal article

  • The Hrq1 helicase stimulates Pso2 translesion nuclease activity to promote DNA inter-strand crosslink repair

    • Authors : Rogers, Cody M; Lee, Chun-Ying; Parkins, Samuel (2020)

  • DNA inter-strand crosslink (ICL) repair requires a complicated network of DNA damage response pathways. Removal of these lesions is vital as they are physical barriers to essential DNA processes that require the separation of duplex DNA, such as replication and transcription. The Fanconi anemia (FA) pathway is the principle mechanism for ICL repair in metazoans and is coupled to replication. In Saccharomyces cerevisiae, a degenerate FA pathway is pr...
  • Journal article

  • Single-particle view of stress-promoters induction dynamics: an interplay between MAPK signaling, chromatin and transcription factors

    • Authors : Bamberger, Casimir; Pankow, Sandra; Bartolomé, Salvador Martínez (2020)

  • Precise regulation of gene expression in response to environmental changes is crucial for cell survival, adaptation and proliferation. In eukaryotic cells, extracellular signal integration is often carried out by Mitogen-Activated Protein Kinases (MAPK). Despite a robust MAPK signaling activity, downstream gene expression can display a great variability between single cells. Using a live mRNA reporter, we monitored the dynamics of transcription in Saccharomyces cerevisiae&#...