Tìm kiếm nâng cao

Current filters:

Current filters:

Discover

Search Results

Item hits:
  • Professional Practice Paper

  • Extracellular Vesicles Analysis in the COVID-19 Era: Insights on Serum Inactivation Protocols Towards Downstream Isolation and Analysis

    • Authors : Frigerio, Roberto (2020)

  • Since the outbreak of COVID-19 crisis, the handling of biological samples from confirmed or suspected SARS-CoV-2 positive individuals demanded the use of inactivation protocols to ensure laboratory operators safety. While not standardized, these practices can be roughly divided in two categories, namely heat inactivation and solvent-detergent treatments. As such, these routine procedures should also apply to samples intended for Extracellular Vesicles (EVs) analysis. Assess...
  • Professional Practice Paper

  • Protein substrates engage the lumen of O-GlcNac transferase’s tetratricopeptide repeat domain in different ways

    • Authors : Joiner, Cassandra M. (2020)

  • Glycosylation of nuclear and cytoplasmic proteins is an essential post-translational modification in mammals. O-GlcNAc transferase (OGT), the sole enzyme responsible for this modification, glycosylates over a thousand unique nuclear and cytoplasmic substrates. How OGT selects its substrates is a fundamental question that must be answered to understand OGT’s unusual biology. OGT contains a long tetratricopeptide repeat (TPR) domain that has been implicated in substrate ...
  • Professional Practice Paper

  • Engineered pH-Sensitive Protein G / IgG Interaction

    • Authors : Jha, Ramesh K. (2020)

  • While natural protein-protein interactions have evolved to be induced by complex stimuli, rational design of interactions that can be switched-on-demand still remain challenging in the protein design world. Here, we demonstrate a computationally redesigned natural interface for improved binding affinity could further be mutated to adopt a pH switchable interaction. The redesigned interface of Protein G-IgG Fc domain, when incorporated with histidine and glutamic acid&#...
  • Periodicals (Báo – Tạp chí)

  • De novo 3D models of SARS-CoV-2 RNA elements and smallmolecule-binding RNAs to aid drug discovery

    • Authors : Rangan, Ramya (2020)

  • he rapid spread of COVID-19 is motivating development of antivirals targeting conserved SARS-CoV-2 molecular machinery. The SARS-CoV-2 genome includes conserved RNA elements that offer potential small-molecule drug targets, but most of their 3D structures have not been experimentally characterized. Here, we provide a compilation of chemical mapping data from our and other labs, secondary structure models, and 3D model ensembles based on Rosetta’s FARFAR2 algorithm ...
  • Periodicals (Báo – Tạp chí)

  • The RNA-binding protein Nab2 regulates the proteome of the developing Drosophila brain

    • Authors : Corgiat, Edwin B. (2020)

  • The human ZC3H14 gene, which encodes a ubiquitously expressed polyadenosine zinc finger RNA binding protein, is mutated in an inherited form of autosomal recessive, non-syndromic intellectual disability. To gain insight into ZC3H14 neurological functions, we previously developed a Drosophila melanogaster model of ZC3H14 loss by deleting the fly ortholog, Nab2. Studies in this invertebrate model reveal that Nab2 controls final patterns of neuron projection within f...
  • Periodicals (Báo – Tạp chí)

  • RNA and DNA G-quadruplexes bind to human Dicer and inhibit its activity

    • Authors : Koralewska, Natalia (2020)

  • Guanine (G)-rich single-stranded nucleic acids can adopt G-quadruplex structures. Accumulating evidence indicates that G-quadruplexes serve important regulatory roles in fundamental biological processes such as DNA replication, transcription, and translation, while aberrant G-quadruplex formation is linked to genome instability and cancer. Understanding the biological functions played by G-quadruplexes requires detailed knowledge of their protein interactome. Here, we report that both RNA ...
  • Periodicals (Báo – Tạp chí)

  • Molecular Elucidation of Pancreatic Elastase Inhibition by Baicalein

    • Authors : Ghosh, Debajeet (2020)

  • The serine protease, elastase exists in various forms and plays diverse roles in the human body. Pharmacological inhibition of elastase has been investigated for its therapeutic role in managing conditions such as diabetes, pneumonia and arthritis. Sivelestat, a synthetic molecule, is the only elastase inhibitor to have been approved by any major drug regulatory authority (PMDA, in this case) – but still has failed to attain widespread clinical usag...
  • Periodicals (Báo – Tạp chí)

  • Manipulation of a Cation-π Sandwich Reveals Conformational Flexibility in Phenylalanine Hydroxylase

    • Authors : Arturo, Emilia C. (2020)

  • Phenylalanine hydroxylase (PAH) is an allosteric enzyme responsible for maintaining phenylalanine (Phe) below neurotoxic levels; its failure results in phenylketonuria. Wild type (WT) PAH equilibrates among resting-state RS-PAH) and activated (A-PAH) conformations, whose equilibrium position depends upon allosteric Phe binding to he A-PAH conformation. The RS-PAH conformation of WT rat PAH (rPAH) contains a cation-π sandwich etween Phe80, Arg123, and Arg420, which cannot ex...
  • Periodicals (Báo – Tạp chí)

  • Revealing enzyme functional architecture via high-throughput microfluidic enzyme kinetics

    • Authors : C.J., Markin (2020)

  • Systematic and extensive investigation of enzymes is needed to understand their extraordinary efficiency and meet current challenges in medicine and engineering. We present HT-MEK, a microfluidic platform for high-throughput expression, purification, and characterization of >1500 enzyme variants per experiment. For 1036 mutants of the alkaline phosphatase PafA, we performed >670,000 reactions to determine >5000 kinetic and physical constants for multiple substrates and ...