- Periodicals (Báo – Tạp chí)
Authors : Nielsen, Alexander L. (2020) - Sirtuin 2 (SIRT2) is a protein deacylase enzyme that removes acetyl groups and longer chain acyl groups from post-translationally modified lysine residues. It affects diverse biological functions in the cell and has been considered a drug target in relation to both neurodegenerative diseases and cancer. Therefore, access to well-characterized and robust tool compounds is essential for the continued investigation of the complex functions of this enzyme. Here,&...
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- Periodicals (Báo – Tạp chí)
Authors : Strzelecka, Dominika; Smietanski, Miroslaw (2020) - Chemical modifications enable preparation of mRNAs with augmented stability and translational activity. In this study, we explored how chemical modifications of 5’,3’-phosphodiester bonds in the mRNA body and polyA tail influence the biological properties of eukaryotic mRNA. To obtain modified and unmodified in vitro transcribed mRNAs, we used ATP and ATP analogues modified at the α-phosphate (containing either O-to-S or O-to-BH3 substitutions) and three different ...
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- Journal article
Authors : Chen, Dapeng; Bryden, Wayne A; McLoughlin, Michael (2020) - Characterization of nonvolatile molecules in exhaled breath particles can be used for respiratory disease monitoring and diagnosis. Conventional methods for the collection of nonvolatile molecules in breath heavily rely on the physical properties of exhaled breath particles. Strategies taking advantage of their chemical properties have not yet been explored.
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- Periodicals (Báo – Tạp chí)
Authors : Takeuchi, Koh; Ikeda, Yoshiki; Harada, Ayaka (2020) - Most kinases function with ATP. However, contrary to the prevailing dogma, phosphatidylinositol 5-phosphate 4-kinase β (PI5P4Kβ) utilizes GTP as a primary phosphate donor with a unique binding mode for GTP. Although PI5P4Kβ is evolved from a primordial ATP-utilizing enzyme, PI4P5K, how PI5P4Kβ evolutionarily acquired the GTP preference to function as a cellular GTP sensor remains unclear
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- Journal article
Authors : Kapoor, Iti; Olivares, Philip; Nair, Satish K (2020) - Diffusible small molecule microbial hormones drastically alter the expression profiles of antibiotics and other drugs in actinobacteria. For example, avenolide (a butenolide) regulates production of avermectin, derivatives of which are used in the treatment of river blindness and other parasitic diseases. Butenolides and γ-butyrolactones control production of pharmaceutically important secondary metabolites by binding to TetR family transcriptional repressors.
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- Journal article
Authors : Jiang, Tao; Roux, Amandine; Engilberge, Sylvain (2020) - In this article, the principle of a database aimed at facilitating the understanding of the unique protein nucleating properties of the Crystallophore is presented. A first analysis allows us to compare the efficiency of Tb-Xo4 with the new Lu-Xo4 variant, featuring improved phasing properties. Then, the concept of subset-of-interest is introduced to reveal potential antagonistic/synergistic effects between Tb-Xo4 and physico-chemical parameters of the crystallisation&...
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- Journal article
Authors : Heinzelmann, Germano; Gilson, Michael K (2020) - Absolute binding free energy calculations with explicit solvent molecular simulations can provide estimates of protein-ligand affinities, and thus reduce the time and costs needed to find new drug candidates. However, these calculations can be complex to implement and perform. Here, we introduce the software BAT.py, a Python tool that invokes the AMBER simulation package to fully automate the calculation of binding free energies for a protein with a ...
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- Journal article
Authors : Nassar, Ray F; Chitralia, Praneeth; Rushworth, Roger (2020) - In recent years, there have been significant efforts devoted to countering the challenge of detecting cancer in early stages. Reduced glutathione (GSH) plays an important role in the antioxidant system and is required for the maintenance of the redox status of the cell, defense against free radicals and detoxification of toxic compounds. GSH may be converted to oxidized glutathione (GSSG) during the oxidative stress that it regularly undergoes when&...
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- Journal article
Authors : Mushtaq, Maria; Asad, Muhammad Javaid; Hyder, Muhammad Zeeshan (2020) - Clostridium thermocellum produces an extracellular cellulosome (a multiprotein complex produced by firmicutes bacteria), which, owing to its extracellular location, is open to protease attack. Serine protease inhibitors (serpins) protect bacteria against protease attack. However, their structure and function are poorly characterized. This study identified and amplified the serpin 1270 gene from the C. thermocellum genome. Purified serpins were cloned into the pTXB1 vector u...
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- Journal article
Authors : Yuan Su, Ming; Zoncu, Roberto; Hurley, James H (2020) - Mutation of C9ORF72 is the most prevalent defect in amyotrophic lateral sclerosis (ALS) and frontal temporal degeneration (FTD). Together with hexanucleotide repeat expansion, haploinsufficiency of C9ORF72 contributes to neuronal dysfunction. We determined the structure of the SMCR8-C9orf72-WDR41 complex by cryo-EM. C9orf72 and SMCR8 are both longin-DENN domain proteins, while WDR41 is a beta-propeller protein that binds to SMCR8 such that the whole structure resembles ...
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