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  • Journal article

  • Generalized Michaelis–Menten rate law with time-varying molecular concentrations

    • Authors : Lim, Roktaek (2023)

  • The Michaelis–Menten (MM) rate law has been the dominant paradigm of modeling23 biochemical rate processes for over a century with applications in biochemistry, biophysics,24 cell biology, and chemical engineering. The MM rate law and its remedied form stand on the25 assumption that the concentration of the complex of interacting molecules, at each moment,26 approaches an equilibrium much faster than the molecular concentrations change. Yet, thi...
  • Journal Article

  • The conserved protein CBA1 is required for vitamin B12 uptake in different algal lineages

    • Authors : Sayer, Andrew P. (2023)

  • Microalgae play an essential role in global net primary productivity and global biogeochemical cycling, but despite their phototrophic lifestyle, over half of algal species depend on a supply of the corrinoid vitamin B12 (cobalamin) for growth. This essential organic micronutrient is produced only by a subset of prokaryotic organisms, which implies that for algal species to use this compound, they must first acquire it from external sources. Previous...
  • Journal article

  • Pyroptosis inhibiting nanobodies block Gasdermin D pore formation

    • Authors : Kopp, Anja  (2023)

  • Gasdermin D (GSDMD) is a key mediator of pyroptosis, a pro-inflammatory form of cell death 10 occurring downstream of inflammasome activation as part of the innate immune defence. Upon cleavage by inflammatory caspases, the N-terminal domain of GSDMD forms pores in the plasma membrane resulting in cytokine release and eventually cell death. Targeting GSDMD is an attractive way to dampen inflammation. In this study, six GSDMD targeting nanobodies wer...
  • Journal article

  • Phase Separation and Ageing of Glycine-Rich Protein from Tick Adhesive

    • Authors : Ganar, Ketan A. (2023)

  • Hard ticks feed on their host for multiple days. To ensure firm attachment, they secrete a protein-rich saliva that eventually forms a solid cement cone. The underlying mechanism of this liquid-to-solid transition is not yet understood. This study focuses on the phase transitions of a disordered glycine- rich protein (GRP) that is prominent in tick saliva. We show that GRP undergoes liquid-liquid phase separation via simple coacervatio...
  • Journal Article

  • Evolving spike-protein N-glycosylation in SARS-CoV-2 variants

    • Authors : Baboo, Sabyasachi (2023)

  • It has been three years since SARS-CoV-2 emerged and the world plunged into a “once in a century” pandemic. Since then, multiple waves of infection have swept through the human population, led by variants that were able to evade any acquired immunity. The co-evolution of SARS-CoV-2 variants with human immunity provides an excellent opportunity to study the interaction between viral pathogens and their human hosts. The heavily N-gl...
  • Journal article

  • Methods Optimization for the Expression and Purification of Human Calcium Calmodulin-Dependent Protein Kinase II Alpha

    • Authors : Bolton, Scott C.  (2023)

  • Calcium/calmodulin-dependent protein kinase II (CaMKII) is a complex multifunctional kinase that is highly expressed in central nervous tissues and plays a key regulatory role in the calcium signaling pathway. Despite over 30 years of recombinant expression and characterization studies, CaMKII continues to be investigated for its impact on signaling cooperativity and its ability to bind multiple substrates through its multimeric hub domain. Here we compare an...