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  • Periodicals (Báo – Tạp chí)

  • Revealing enzyme functional architecture via high-throughput microfluidic enzyme kinetics

    • Authors : C.J., Markin (2020)

  • Systematic and extensive investigation of enzymes is needed to understand their extraordinary efficiency and meet current challenges in medicine and engineering. We present HT-MEK, a microfluidic platform for high-throughput expression, purification, and characterization of >1500 enzyme variants per experiment. For 1036 mutants of the alkaline phosphatase PafA, we performed >670,000 reactions to determine >5000 kinetic and physical constants for multiple substrates and ...
  • Ebooks (Sách điện tử)

  • Membrane Proteins: Structure, Function and Motion

    • Authors : - (2023)

  • Cuốn sách này là tập hợp các bài đánh giá và bài nghiên cứu gốc về sinh hóa vật lý của protein màng. Các khía cạnh khác nhau của cấu trúc phức tạp và động và hành vi chức năng của protein màng trong màng tế bào và tương tác của chúng với lipid, protein và các phân tử và ion khác đã được một số nhà khoa học hàng đầu trong lĩnh vực này nghiên cứu. Cuốn sách này hữu ích cho sinh...
  • Journal Article

  • Conformation and structural dynamics of the Xist lncRNA A-repeats

    • Authors : Jones, Alisha N. (2023)

  • Long noncoding RNAs (lncRNAs) are non‐protein‐encoding transcripts with lengths of 500 or more nucleotides (nt), with some spanning up to several thousands of nucleotides 1. LncRNAs have emerged as important regulators of gene expression and cellular functions, as transcriptional regulators at the level of chromatin, as scaffolding platforms, and as guides for RNP localization 2–9. Recent advancements in chemical probing experiments and their couplin...
  • Journal Article

  • MicroED structure of the human vasopressin 1B receptor

    • Authors : Shiriaeva, Anna (2023)

  • Mucin 1 (MUC1) is a transmembrane mucin expressed at the apical surface of epithelial cells at different mucosal surfaces including breast and intestine. In the gastrointestinal tract, MUC1 has a barrier function against bacterial invasion, but can also serve as an entry receptor for pathogenic Salmonella bacteria. Moreover, MUC1 is well known for its aberrant expression and glycosylation in adenocarcinomas The MUC1 extracellular do...
  • Journal article

  • Structure and function of a Notch-like proteolytic switch domain in the extracellular matrix receptor dystroglycan

    • Authors : Anderson, Michael J.M. (2023)

  • The adhesion receptor dystroglycan provides a critical mechanical link between the extracellular matrix (ECM) and the actin cytoskeleton to help muscle cells withstand contraction and neural cells maintain the blood brain barrier. Disrupting the link is associated cancer and muscular dystrophy. Proteolysis of dystroglycan by matrix metalloproteinases (MMPs) provides a mechanism to break the mechanical anchor and is amplified in several pathogenic states,&...
  • Journal article

  • Cryo-EM structures of Na+-pumping NADH-ubiquinone oxidoreductase from Vibrio cholerae

    • Authors : Kishikawa, Jun-ichi (2023)

  • The Na+-pumping NADH-ubiquinone oxidoreductase (Na+-NQR) couples electron transfer 3 from NADH to ubiquinone with Na+-pumping, generating an electrochemical Na+ gradient that 4 is essential for energy-consuming reactions in bacteria. Since Na+-NQR is exclusively found in 5 prokaryotes, it is a promising target for highly selective antibiotics. However, the molecular 6 mechanism of inhibition is not well-understood for lack of the atomic structural informa...
  • Journal Article

  • Structure of full-length cobalamin-dependent methionine synthase and cofactor loading captured in crystallo

    • Authors : Mendoza, Johnny (2023)

  • Cobalamin-dependent methionine synthase (MS) is a key enzyme in methionine and folate one-carbon metabolism. MS is a large multi-domain protein capable of binding and activating three substrates: homocysteine, folate, S-adenosylmethionine for methylation. Achieving three chemically distinct methylations necessitates significant domain rearrangements to facilitate substrate access to the cobalamin cofactor at the right time. The distinct conformations required for each r...
  • Journal Article

  • Structure of the Lysinibacillus sphaericus Tpp49Aa1 pesticidal protein elucidated from natural crystals using MHz-SFX

    • Authors : Williamson, Lainey J.  (2023)

  • Tpp49Aa1 from Lysinibacillus sphaericus is a Toxin_10 family protein that – in combination with Cry48Aa1, a 3-domain crystal protein - has potent mosquitocidal activity, specifically against Culex quinquefasciatus mosquitoes. MHz serial femtosecond crystallography at a nano-focused X-ray free electron laser, allowed rapid and high-quality data collection to determine the Tpp49Aa1 structure at 1.62 Å resolution from native nanocrystals. This revealed the packin...