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  • Journal article

  • Structure of a dimeric ABC transporter

    • Authors : Bickers, Sarah C.  (2023)

  • ATP binding cassette (ABC) proteins generally couple ATP hydrolysis to the active 20 transport of solutes across cellular membranes. All ABC proteins contain a core structure of two 21 transmembrane domains (TMD1, TMD2) and two nucleotide binding domains (NBD1, NBD2), 22 and many ABC proteins contain additional domains. Some members of the C subfamily of ABC 23 (ABCC) proteins, such as the multidrug resistant protein 1 (MRP1), con...
  • Journal article

  • Structural basis for reduced ribosomal A-site fidelity in response to P-site codon-anticodon mismatches

    • Authors : Nguyen, Ha An (2023)

  • Rapid and accurate translation is essential in all organisms to produce properly folded and functional proteins. mRNA codons that define the protein coding sequences are decoded by tRNAs on the ribosome in the aminoacyl (A) binding site. The mRNA codon and the tRNA anticodon interaction is extensively monitored by the ribosome to ensure accuracy in tRNA selection. While other polymerases that synthesize DNA and RNA can correct for...
  • Journal article

  • Structure of a Membrane Tethering Complex Incorporating Multiple SNAREs

    • Authors : DAmico, Kevin A. (2023)

  • Most membrane fusion reactions in eukaryotic cells are mediated by membrane tethering 17 complexes (MTCs) and SNARE proteins. MTCs are much larger than SNAREs and are thought 18 to mediate the initial attachment of two membranes. Complementary SNAREs then form 19 membrane-bridging complexes whose assembly draws the membranes together for fusion. Here, 20 we present a cryo-EM structure of the simplest known MTC, the 255-kDa Dsl1 complex...
  • Ebooks (Sách điện tử)

  • Structural insights into the initiation of free radical formation in the Class Ib ribonucleotide reductases

    • Authors : Yadav, Lumbini R. (2023)

  • Class I ribonucleotide reductases consisting of R1 and R2 subunits convert ribonucleoside diphosphates to deoxyribonucleoside diphosphates involving an intricate free radical mechanism. The generation of free radicals in the Class Ib ribonucleotide reductases is mediated by reaction involving di-manganese ions in the R2 subunits and is externally assisted by flavodoxin-like NrdI subunit. This is unlike Class Ia ribonucleotide reductases, where the free radical generation&#x...
  • Journal article

  • Structure and function of a hexameric cyanophycin synthetase 2

    • Authors : Markus, Linda M. D.  (2023)

  • Cyanophycin is a natural polymer composed of a poly-aspartate backbone with arginine attached to each of the aspartate sidechains. Produced by a wide range of bacteria, which mainly use it as a store of fixed nitrogen, it has many promising industrial applications. Cyanophycin can be synthesized from the amino acids Asp and Arg by the widespread cyanophycin synthetase 1 (CphA1), or from the dipeptide β-Asp-Arg by the cyanobacteria...
  • Ebooks (Sách điện tử)

  • The structure of the neurotoxin palytoxin determined by MicroED

    • Authors : Gillman, Cody (2023)

  • Palytoxin (PTX) is a potent neurotoxin found in marine animals that can cause serious symptoms 17 such as muscle contractions, haemolysis of red blood cells and potassium leakage. Despite years 18 of research, very little is known about the mechanism of PTX. However, recent advances in the 19 field of cryoEM, specifically the use of microcrystal electron diffraction (MicroED), have allowed 20 us to determine the structure of PTX. It was&#x...
  • Journal Article

  • Structure of the Lysinibacillus sphaericus Tpp49Aa1 pesticidal protein elucidated from natural crystals using MHz-SFX

    • Authors : Williamson, Lainey J.  (2023)

  • Tpp49Aa1 from Lysinibacillus sphaericus is a Toxin_10 family protein that – in combination with Cry48Aa1, a 3-domain crystal protein - has potent mosquitocidal activity, specifically against Culex quinquefasciatus mosquitoes. MHz serial femtosecond crystallography at a nano-focused X-ray free electron laser, allowed rapid and high-quality data collection to determine the Tpp49Aa1 structure at 1.62 Å resolution from native nanocrystals. This revealed the packin...
  • Journal article

  • An alphacoronavirus polymerase structure reveals conserved co-factor functions

    • Authors : Anderson, Thomas K. (2023)

  • Coronaviruses are a diverse subfamily of viruses containing pathogens of humans and animals. This subfamily of viruses replicates their RNA genomes using a core polymerase complex composed of viral non-structural proteins: nsp7, nsp8 and nsp12. Most of our understanding of coronavirus molecular biology comes from the betacoronaviruses like SARS-CoV and SARSCoV- 2, the latter of which is the causative agent of COVID-19. In...
  • Journal article

  • Structural analysis of SALL4 zinc-finger domain reveals a link between AT-rich DNA binding and Okihiro syndrome

    • Authors : Watson, James A. (2023)

  • Spalt-like 4 (SALL4) maintains vertebrate embryonic stem cell identity and is required for the development of multiple organs, including limbs. Mutations in SALL4 are associated with Okihiro syndrome and SALL4 is also a known target of thalidomide. SALL4 protein has a distinct preference for AT-rich sequences, recognised by a pair of zinc fingers at the C-terminus. However, unlike many characterised zinc finger proteins, SALL4 shows flexibl...
  • Journal article

  • CryoEM Structures of the Nitrogenase Complex During Catalytic Turnover

    • Authors : Rutledge, Hannah L. (2023)

  • The enzyme nitrogenase couples adenosine triphosphate (ATP) hydrolysis to the multi-electron reduction of atmospheric dinitrogen into ammonia. Despite extensive research, the mechanistic details of ATP-dependent energy transduction and dinitrogen reduction by nitrogenase are not well understood, requiring new strategies to monitor its structural dynamics during catalytic action. Here we report the cryogenic electron microscopic interrogation of the nitrogenase complex under enzymatic ...