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  • Journal article

  • An alphacoronavirus polymerase structure reveals conserved co-factor functions

    • Authors : Anderson, Thomas K. (2023)

  • Coronaviruses are a diverse subfamily of viruses containing pathogens of humans and animals. This subfamily of viruses replicates their RNA genomes using a core polymerase complex composed of viral non-structural proteins: nsp7, nsp8 and nsp12. Most of our understanding of coronavirus molecular biology comes from the betacoronaviruses like SARS-CoV and SARSCoV- 2, the latter of which is the causative agent of COVID-19. In...
  • Journal article

  • CryoEM Structures of the Nitrogenase Complex During Catalytic Turnover

    • Authors : Rutledge, Hannah L. (2023)

  • The enzyme nitrogenase couples adenosine triphosphate (ATP) hydrolysis to the multi-electron reduction of atmospheric dinitrogen into ammonia. Despite extensive research, the mechanistic details of ATP-dependent energy transduction and dinitrogen reduction by nitrogenase are not well understood, requiring new strategies to monitor its structural dynamics during catalytic action. Here we report the cryogenic electron microscopic interrogation of the nitrogenase complex under enzymatic ...
  • Journal Article

  • Structure and function of a YeeE-YeeD complex for sophisticated thiosulfate uptake

    • Authors : Ikei, Mai (2023)

  • From bacteria to eukaryotes, sulfur is a vital element for cellular activities. For example, sulfur34 containing biomolecules, such as L-cysteine, L-methionine, thiamine, glutathione, and biotin, play a 35 variety of essential roles in cells (1). Bacteria and plants can utilize L-cysteine as a source of sulfate, 36 but they also have sulfur assimilation pathways to synthesize L-cysteine from inorganic sulfur 37 compounds. In bacteria, L-cysteine...
  • Journal article

  • Structure of a Membrane Tethering Complex Incorporating Multiple SNAREs

    • Authors : DAmico, Kevin A. (2023)

  • Most membrane fusion reactions in eukaryotic cells are mediated by membrane tethering 17 complexes (MTCs) and SNARE proteins. MTCs are much larger than SNAREs and are thought 18 to mediate the initial attachment of two membranes. Complementary SNAREs then form 19 membrane-bridging complexes whose assembly draws the membranes together for fusion. Here, 20 we present a cryo-EM structure of the simplest known MTC, the 255-kDa Dsl1 complex...