Journal articleAuthors : Fleming, Aaron M. (2021)
Nanopore devices can directly sequence RNA, and the method has the potential to determine
locations of epitranscriptomic modifications that have grown in significance because of their roles
in cell regulation and stress response. Pseudouridine (Ψ), the most common modification in RNA,
was sequenced with a nanopore system using a protein sensor with a helicase brake in synthetic
RNAs with 100% modification at 18 known human pseudouridinylati...