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  • Journal article

  • Structural probing of Hsp26 activation and client binding by quantitative cross-linking mass spectrometry

    • Authors : Fürsch, Julius (2021)

  • Small heat-shock proteins (sHSP) are important members of the cellular stress response in all species. Their best described function is the binding of early unfolding states and the resulting prevention of protein aggregation. Most sHSPs exist as oligomers but vary in size and subunit organization. Many sHSPs exist as a polydisperse composition of oligomers which undergoes changes in subunit composition, folding status and rel...
  • Journal article

  • Structure Activity Relationship of USP5 Allosteric Inhibitors

    • Authors : Mann, Mandeep K (2021)

  • USP5 is a deubiquitinase that has been implicated in a range of diseases, including cancer, but no USP5- targeting chemical probe has been reported to date. Here, we present the progression of a chemical series that occupies the C-terminal ubiquitin-binding site of a poorly characterized zinc-finger ubiquitin binding domain (ZnF-UBD) of USP5 and allosterically inhibits the catalytic activity of the enzyme. Systematic exploration of&...
  • Journal article

  • Considerations on activity determinants of fungal polyphenol oxidases based on mutational and structural studies

    • Authors : Nikolaivits, Efstratios (2021)

  • Polyphenol oxidases (PPOs) are an industrially relevant family of enzymes, being involved in the post-harvest browning of fruits and vegetables, as well as in human melanogenesis. Their involvement lies in their ability to oxidize phenolic or polyphenolic compounds, that subsequently form pigments. PPO family includes tyrosinases and catechol oxidases, which in spite of their high structural similarity, exhibit different catalytic activities. Long-standing research efforts&...
  • Journal article

  • Nanopore dwell time analysis permits sequencing and conformational assignment of pseudouridine in SARS-CoV-2

    • Authors : Fleming, Aaron M. (2021)

  • Nanopore devices can directly sequence RNA, and the method has the potential to determine locations of epitranscriptomic modifications that have grown in significance because of their roles in cell regulation and stress response. Pseudouridine (Ψ), the most common modification in RNA, was sequenced with a nanopore system using a protein sensor with a helicase brake in synthetic RNAs with 100% modification at 18 known human pseudouridinylati...