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  • Journal Article

  • Structure-guided engineering of biased-agonism in the human niacin receptor via single amino acid substitution

    • Authors : Yadav, Manish K. (2023)

  • The Hydroxycarboxylic acid receptor 2 (HCA2), also known as the niacin receptor or GPR109A, is a prototypical G protein-coupled receptor that plays a central role in the inhibition of lipolytic and atherogenic activities in our body. Interestingly, GPR109A activation also results in vasodilation that is linked to the side-effect of flushing associated with dyslipidemia drugs such as niacin. This receptor continues to be a key target for developing n...
  • Journal Article

  • Structural rather than catalytic role for mitochondrial respiratory chain supercomplexes

    • Authors : Brischigliaro, Michele (2023)

  • Mammalian mitochondrial respiratory chain (MRC) complexes are able to associate into quaternary structures named supercomplexes (SCs), which normally coexist with non bound individual complexes. The functional significance of SCs has not been fully clarified and the debate has been centered on whether or not they confer catalytic advantages to the non-bound individual complexes. Mitochondrial respiratory chain organization does not seem to be ...
  • Ebooks (Sách điện tử)

  • Structural Characterization of the Full-length Hantaan Virus Polymerase

    • Authors : keown, Jeremy R (2023)

  • Hantaviridae are a family of segmented negative-sense RNA viruses that contains important human and animal pathogens including Andes orthohantavirus and Hantaan orthohantavirus. Hantaviridae contain a viral RNA-dependent RNA polymerase that replicates and transcribes the viral genome in the cytoplasm of infected cells. Here we establish the expression and purification of the polymerase from the Old World Hantaan orthohantavirus and characterise the structure&#...
  • Journal article

  • Antisense pairing and SNORD13 structure guide RNA cytidine acetylation

    • Authors : Gamage, Supuni Thalalla (2023)

  • N4-acetylcytidine (ac4C) is an RNA nucleobase found in all domains of life. Establishment of ac4C in helix 45 (h45) of human 18S ribosomal RNA (rRNA) requires the combined activity of the acetyltransferase NAT10 and the box C/D snoRNA SNORD13. However, the molecular mechanisms governing RNA-guided nucleobase acetylation in humans remain unexplored. Here we report two assays that enable the study of SNORD13-dependent RNA acetylation in human cells. F...
  • Journal article

  • Structural Insights into the Iron Nitrogenase Complex

    • Authors : Schmidt, Frederik V.  (2023)

  • Nitrogenases are best known for catalysing the reduction of dinitrogen to ammonia at a complex metallic cofactor. Recently, nitrogenases were shown to reduce carbon dioxide (CO2) and carbon monoxide to hydrocarbons, offering a pathway to recycle carbon waste into hydrocarbon products. Among the nitrogenase family the iron nitrogenase is the isozyme with the highest wildtype activity for the reduction of CO2, but the molecular architecture facilitating these&#...
  • Journal article

  • Structural insights into perilipin 3 membrane association in response to diacylglycerol accumulation

    • Authors : Choi, Yong Mi (2023)

  • Lipid droplets (LDs) are dynamic organelles that contain an oil core mainly composed of 36 triglycerides (TAG) that is surrounded by a phospholipid monolayer and LD-associated proteins 37 called perilipins (PLINs). During LD biogenesis, perilipin 3 (PLIN3) is recruited to nascent LDs as 38 they emerge from the endoplasmic reticulum. Here, we analyzed how lipid composition affects 39 PLIN3 recruitment to membrane bilayers and LDs, and t...