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  • Journal Article

  • Structural rather than catalytic role for mitochondrial respiratory chain supercomplexes

    • Authors : Brischigliaro, Michele (2023)

  • Mammalian mitochondrial respiratory chain (MRC) complexes are able to associate into quaternary structures named supercomplexes (SCs), which normally coexist with non bound individual complexes. The functional significance of SCs has not been fully clarified and the debate has been centered on whether or not they confer catalytic advantages to the non-bound individual complexes. Mitochondrial respiratory chain organization does not seem to be ...
  • Journal Article

  • Shared Structural Features of Miro Binding Control Mitochondrial Homeostasis

    • Authors : Covill-Cooke, Christian (2023)

  • Miro proteins are universally conserved mitochondrial calcium-binding GTPases that regulate a multitude of mitochondrial processes, including transport, clearance and lipid trafficking. Miro binds a variety of client proteins involved in these functions. How this binding is operated at the molecular level and whether and how it is important for mitochondrial health, however, remains unknown. Here, we show that known Miro clients all use a similar short motif&...
  • Journal Article

  • Application of monolayer graphene to cryo-electron microscopy grids for high-resolution structure determination

    • Authors : Grassetti, andrew V (2023)

  • The application of support layers, such as graphene, to cryo-electron microscopy grids can increase the density of particles imaged, limit particle interactions with the air-water interface, reduce the extent of beam-induced motion, and, in some instances, improve the distribution of particle orientations. This paper describes a robust protocol for coating cryo-EM grids with a monolayer of graphene for improved cryo-sample preparation. Single particle cryo...
  • Journal article

  • Uncovering the structural flexibility of SARS-CoV-2 glycoprotein spike variants

    • Authors : Arruda, Hiam R. S. (2023)

  • The severe acute respiratory syndrome CoV-2 rapidly spread worldwide, causing a 43 pandemic. After a period of evolutionary stasis, a set of SARS-CoV-2 mutations has arisen in the 44 spike, the leading glycoprotein at the viral envelope and the primary antigenic candidate for 45 vaccines against the 2019 CoV disease (COVID-19). Here, we present comparative biochemical 46 data of the glycosylated full-length ancestral and D614G spike to...
  • Journal article

  • Conserved Structure Modules within the lncRNA SChLAP1 Mediate Protein Recognition Implicated in Aggressive Prostate Cancer

    • Authors : McFadden, Emily J. (2023)

  • The lncRNA Second Chromosome Locus Associated with Prostate 1 (SChLAP1) was previously identified as a predictive biomarker and potential driver of aggressive prostate cancer. Recent work suggests that SChLAP1 may bind the SWI/SNF chromatin remodeling complex to promote prostate cancer metastasis, though the exact role of SWI/SNF recognition is debated. To date, there are no detailed biochemical studies of apo SChLAP1 or SChLAP1:protein com...
  • Journal article

  • Structural insights into agonist-binding and activation of the human complement C3a receptor

    • Authors : Yadav, Manish K. (2023)

  • The complement cascade is an integral part of innate immunity, and it plays a crucial role in our body's innate immune response including combating microbial infections. Activation of the complement cascade results in the generation of multiple peptide fragments, of which complement C3a and C5a are potent anaphylatoxins. The complement C3a binds and activates a G protein-coupled receptor (GPCR) known as C3aR while C5a activates two distinct receptors...
  • Journal article

  • Sensitive detection and structural characterisation of UV-induced cross-links in protein-RNA complexes using CLIR-MS

    • Authors : Sarnowski, Chris P. (2023)

  • Cross-linking coupled with mass spectrometry is an increasingly popular methodology for elucidating structural information from biological complexes. Whilst protein-protein cross-linking workflows are widely used and well characterised, adoption of protein-RNA cross-linking workflows for structural studies is less widespread, and data produced from such experiments remains less well understood. The cross-linking of stable isotope labelled RNA coupled to mass spectrometry (CLIR-MS) workflo...