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  • Journal article

  • Phase Separation and Ageing of Glycine-Rich Protein from Tick Adhesive

    • Authors : Ganar, Ketan A. (2023)

  • Hard ticks feed on their host for multiple days. To ensure firm attachment, they secrete a protein-rich saliva that eventually forms a solid cement cone. The underlying mechanism of this liquid-to-solid transition is not yet understood. This study focuses on the phase transitions of a disordered glycine- rich protein (GRP) that is prominent in tick saliva. We show that GRP undergoes liquid-liquid phase separation via simple coacervatio...
  • Journal Article

  • Evolving spike-protein N-glycosylation in SARS-CoV-2 variants

    • Authors : Baboo, Sabyasachi (2023)

  • It has been three years since SARS-CoV-2 emerged and the world plunged into a “once in a century” pandemic. Since then, multiple waves of infection have swept through the human population, led by variants that were able to evade any acquired immunity. The co-evolution of SARS-CoV-2 variants with human immunity provides an excellent opportunity to study the interaction between viral pathogens and their human hosts. The heavily N-gl...
  • Journal article

  • Computational design of Matrix Metalloprotenaise-9 (MMP-9) resistant to auto-cleavage

    • Authors :  Bonadio, Alessandro (2023)

  • Matrix metalloproteinase-9 (MMP-9) is an endopeptidase that remodels the extracellular matrix and has been implicated as a major driver in cancer metastasis. Hence, there is a high demand for MMP-9 inhibitors for therapeutic purposes. For such drug design efforts, large amounts of MMP-9 are required. Yet, the catalytic domain of MMP-9 (MMP-9Cat) is an intrinsically unstable enzyme that tends to auto-cleave within minutes, making it di...
  • Journal article

  • Structural probing of Hsp26 activation and client binding by quantitative cross-linking mass spectrometry

    • Authors : Fürsch, Julius (2021)

  • Small heat-shock proteins (sHSP) are important members of the cellular stress response in all species. Their best described function is the binding of early unfolding states and the resulting prevention of protein aggregation. Most sHSPs exist as oligomers but vary in size and subunit organization. Many sHSPs exist as a polydisperse composition of oligomers which undergoes changes in subunit composition, folding status and rel...
  • Journal Article

  • Borrelia PeptideAtlas: A proteome resource of common Borrelia burgdorferi isolates for Lyme research

    • Authors : Reddy, Panga Jaipal. (2023)

  • Lyme disease , caused by an infection with the spirochete Borrelia burgdorferi, is the most common 36 vector-borne disease in North America. B. burgdorferi strains harbor extensive genomic and 37 proteomic variability and further comparison is key to understanding the spirochetes infectivity and 38 biological impacts of identified sequence variants. To achieve this goal, both transcript and mass 39 spectrometry (MS)-based proteomics was applied to assemble pe...
  • Journal Article

  • Type III-B CRISPR-Cas signaling-based cascade of proteolytic cleavages

    • Authors : Steens, Jurre A. (2023)

  • Type III CRISPR-Cas systems provide a sequence-specific adaptive immune response that protects prokaryotic hosts against viruses and other foreign genetic invaders. These crRNA-guided Cas effector complexes bind and cleave complementary RNA targets. Specific target binding stimulates the Cas10 subunit to generate cyclic oligoadenylate (cOA) signaling molecules, that in turn allosterically activate proteins carrying cognate sensory domains: CARF or SAVED. Here, we characterize an...
  • Journal article

  • TDFragMapper: a visualization tool for evaluating experimental parameters in top-down proteomics

    • Authors : Dhenin, Jonathan (2021)

  • Top-down proteomics (TDP) is a powerful technology allowing the char acterization of proteins at the proteoform level using high-resolution tan dem mass spectrometry (MS/MS). Proteoforms correspond to the different forms of a protein arising from all combinatorial sources of variation from a single gene (including combinations of genetic variation, alternative splicing, and post-translational modifications)(Smith and Kelleher, 2018). The complete...