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  • Journal article

  • Sulfonated rhodamines as impermeable labelling substrates for cell surface protein visualization

    • Authors : Birke, Ramona (2021)

  • Sulfonated rhodamines that endow xanthene dyes with cellular impermeability are presented. We fuse charged sulfonates to red and far-red dyes to obtain Sulfo549 and Sulfo646, respectively, and further link these to SNAP- and Halo-tag substrates for protein self-labelling. Cellular impermeability is validated in live cell imaging experiments in transfected HEK cells and neurons derived from induced pluripotent stem cells (iPSCs). Lastly, we show that&...
  • Journal article

  • AMPylation is a specific lysosomal protein posttranslational modification in neuronal maturation

    • Authors :  Becker, Tobias (2021)

  • Protein AMPylation is a pervasive posttranslational modification with an emerging role in neurodevelopment. In metazoans the two highly conserved protein AMP-transferases together with a diverse group of AMPylated proteins have been identified using chemical proteomics and biochemical techniques. However, the function of this modification remains largely unknown. Particularly problematic is the localization of thus far identified AMPylated proteins and putative&#x...
  • Journal Article

  • A global, integrated view of the ubiquitylation site occupancy and dynamics

    • Authors : Prus, Gabriela (2023)

  • Ubiquitylation regulates virtually all proteins and biological processes in a cell. However, the global site-specific occupancy (stoichiometry) and turnover rate of ubiquitylation have never been quantified. Here, we present the first integrated picture of ubiquitylation site occupancy and half-life. Ubiquitylation occupancy spans four orders of magnitude, but the median ubiquitylation site occupancy is three orders of magnitude lower than that of 20...