OER - Kỹ thuật hóa học; Công nghệ sinh học - Thực phẩm; Công nghệ môi trường (1155)



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Collection's Items (Sorted by Submit Date in Descending order): 1 to 20 of 1155

  • OER000000750.pdf.jpg
  • Journal article


  • Authors : Kenta, Arai; Kazuya, Kabayama; Yoshimi, Kanie (2020)

  • Glycolipid metabolism occurs in the Golgi apparatus, but the detailed mechanisms have not yet been elucidated. We used fluorescently labeled glycolipids to analyze glycolipid composition and localization changes and shed light on glycolipid metabolism. In a previous study, the fatty chain of lactosyl ceramide was fluorescently labeled with BODIPY (LacCer-BODIPY) before being introduced into cultured cells to analyze the cell membrane glycolipid recycling process.

  • OER000000749.pdf.jpg
  • Journal article


  • Authors : Mullally, Grace; Aelst, Kara van; Naqvi, Mohsin M (2020)

  • A key aim in exploiting CRISPR-Cas is the engineering of gRNA to introduce additional functionalities, ranging from small nucleotide changes that increase efficiency of on-target binding to the inclusion of large functional RNA aptamers and ribonucleoproteins (RNPs. Interactions between gRNA and Cas9 are crucial for RNP complex assembly but several distinct regions of the gRNA are amenable to modification. Using a library of modified gRNAs, we used in&#x...

  • OER000000748.pdf.jpg
  • Journal article


  • Authors : Pastor, Manuel Martín; Codeseira, Yaiza B; Spagnolli, Giovanni (2020)

  • PrPSc, the first described and most notorious prion, is the only protein known to cause epidemics of deadly disease. Its properties are encoded in its unique structure. Here we report a first solid state NMR study of a uniformly labelled (U-13C,15N)-Bank vole (BV) infectious recombinant PrPSc prion. C-C, C-H and N-H spectra were obtained with MAS rotation of the sample at up to 60 kHz. We obtained amino acid-type secondary structure i...

  • OER000000745.pdf.jpg
  • Journal article


  • Authors : Scaiola, Alain; Mangia, Francesca; Imseng, Stefan (2020)

  • The protein kinase mammalian target of rapamycin (mTOR) is the central regulator of cell growth. Aberrant mTOR signaling is linked to cancer, diabetes and neurological disorders. mTOR exerts its functions in two distinct multiprotein complexes, mTORC1 and mTORC2.

  • OER000000744.pdf.jpg
  • Journal article


  • Authors : Zhang, Qi; Aguilera, Javier (2020)

  • EsxA has long been recognized as an important virulence factor of Mycobacterium tuberculosis (Mtb) that plays an essential role in Mtb cytosolic translocation presumably by penetrating phagosomal membranes with its acidic pH-dependent membrane permeabilizing activity (MPA). However, current data suggest that the observed cytolytic activity of EsxA at neutral pH is due to contamination of ASB-14, a detergent used in EsxA protein purification, and the role of&#...

  • OER000000743.pdf.jpg
  • Journal article


  • Authors : Meiser, Johannes; Vazquez, Alexei (2020)

  • The Pasteur effect dictates that oxygen induces respiration and represses fermentation. However, we have shown that oxygen stimulates mitochondrial formate production and excess formate production induces glycolysis in mammalian cells. Our observations suggest the hypothesis that increased respiration induces an increase, rather than a decrease, of fermentation, the reverse Pasteur effect. Using a mathematical model we show that, in the absence of mitochondrial formate ...

  • OER000000742.pdf.jpg
  • Journal article


  • Authors : Lobel, Joseph H; Gross, John D (2020)

  • Pat1 promotes the activation and assembly of multiple proteins during mRNA decay. After deadenylation, the Pat1/Lsm1-7 complex binds to transcripts containing oligo(A) tails, which can be modified by the addition of several terminal uridine residues. Pat1 enhances Lsm1-7 binding to the 3’ end, but it is unknown how this interaction is influenced by nucleotide composition. Here we examine Pat1/Lsm1-7 binding to a series of oligoribonucleotides containing&...

  • OER000000741.pdf.jpg
  • Journal article


  • Authors : Orsburn, Benjamin C; Jenkins, Conor; Miller, Sierra M (2020)

  • We describe a method for rapid in silico selection of diagnostic peptides from newly described viral pathogens and applied this approach to SARS-CoV-2/COVID-19. This approach is multi-tiered, beginning with compiling the theoretical protein sequences from genomic derived data. In the case of SARS-CoV-2 we begin with 496 peptides that would be produced by proteolytic digestion of the viral proteins. To eliminate peptides that would cause cross-reactivity ...

  • OER000000740.pdf.jpg
  • Journal article


  • Authors : Kemp, Grant; Nilsson, Ola B; Tian, Pengfei (2020)

  • Proteins synthesized in the cell can begin to fold during translation before the entire polypeptide has been produced, which may be particularly relevant to the folding of multidomain proteins. Here, we study the cotranslational folding of adjacent domains from the cytoskeletal protein α-spectrin using Force Profile Analysis (FPA).

  • OER000000739.pdf.jpg
  • Journal article


  • Authors : Yuan Su, Ming; Zoncu, Roberto; Hurley, James H (2020)

  • Mutation of C9ORF72 is the most prevalent defect in amyotrophic lateral sclerosis (ALS) and frontal temporal degeneration (FTD). Together with hexanucleotide repeat expansion, haploinsufficiency of C9ORF72 contributes to neuronal dysfunction. We determined the structure of the SMCR8-C9orf72-WDR41 complex by cryo-EM. C9orf72 and SMCR8 are both longin-DENN domain proteins, while WDR41 is a beta-propeller protein that binds to SMCR8 such that the whole structure resembles ...

  • OER000000738.pdf.jpg
  • Journal article


  • Authors : Fos, Santi Mestre; Ito, Chieri; Moore, Courtney M (2020)

  • The in vitro formation of stable G-quadruplexes (G4s) in human ribosomal RNA (rRNA) was recently reported. However, their formation in cells and their cellular roles have not been resolved. Here, by taking a chemical biology approach that integrates results from immunofluorescence, G4 ligands, heme affinity reagents, and a genetically encoded fluorescent heme sensor, we report that human ribosomes can form G4s in vivo that regulate heme bioavailability. ...

  • OER000000737.pdf.jpg
  • Journal article


  • Authors : Oda, Toshiyuki; Yanagisawa, Haruaki (2020)

  • The Z-disc forms a boundary between sarcomeres, which constitute structural and functional units of striated muscle tissue. Actin filaments from adjacent sarcomeres are cross-bridged by α-actinin in the Z-disc, allowing transmission of tension across the myofibril. Despite decades of studies, the 3D structure of Z-disc has been elusive due to the limited resolution of conventional electron microscopy.

  • OER000000736.pdf.jpg
  • Journal article


  • Authors : Norman, Brendan P; Davison, Andrew S; Hughes, Juliette H (2020)

  • Background and Purpose alkaptonuria (AKU) is an inherited disorder of tyrosine metabolism caused by lack of the enzyme homogentisate 1,2-dioxygenase (HGD). The primary biochemical consequence of HGD-deficiency is increased circulating homogentisic acid (HGA), which is central to AKU disease pathology. The aim of this study was to investigate the wider metabolic consequences of targeted Hgd disruption. Experimental Approach the first metabolomic analysis of the Hg...

  • OER000000735.pdf.jpg
  • Journal article


  • Authors : Heinzelmann, Germano; Gilson, Michael K (2020)

  • Absolute binding free energy calculations with explicit solvent molecular simulations can provide estimates of protein-ligand affinities, and thus reduce the time and costs needed to find new drug candidates. However, these calculations can be complex to implement and perform. Here, we introduce the software BAT.py, a Python tool that invokes the AMBER simulation package to fully automate the calculation of binding free energies for a protein with a ...

  • OER000000734.pdf.jpg
  • Journal article


  • Authors : Jakubec, Martin; Grødem, Jodi Maple; Akbari, Saleha (2020)

  • Exosomes are vesicles involved in intercellular communication. Their membrane structure and core content is largely dependent on the cell of origin. Exosomes have been investigated both for their biological roles and their possible use as disease biomarkers and drug carriers. These potential technological applications require the rigorous characterization of exosomal blood brain barrier permeability and a description of their lipid bilayer composition. To achieve these...

  • OER000000731.pdf.jpg
  • Journal article


  • Authors : Nakano, Takanari; Inoue, Ikuo; Takenaka, Yasuhiro (2020)

  • Ezetimibe inhibits Niemann-Pick C1-like 1 (NPC1L1) protein, which mediates intracellular cholesterol trafficking from the brush border membrane to the endoplasmic reticulum, where chylomicron assembly takes place in enterocytes or in the intestinal absorptive epithelial cells. Cholesterol is a minor lipid component of chylomicrons; however, whether or not a shortage of cholesterol attenuates chylomicron assembly is unknown. The aim of this study was to examine the ...