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Title: Na+/H+ antiporter activity by respiratory complex I controls mitochondrial Δψ and is impaired in LHON disease
Authors: Agustín, Pablo Hernansanz
Keywords: chống phản ứng; Na+/H+; phức hợp hô hấp; kiểm soát; bệnh LHON
Issue Date: 2023
Publisher: bioRxiv
Abstract: The mitochondrial electron transport chain (mETC) converts the energy of substrate oxidation into a H+ electrochemical gradient (Δp), which is composed by an inner mitochondrial membrane (IMM) potential (ΔΨmt) and a pH gradient (ΔpH). So far, ΔΨmt has been assumed to be composed exclusively by H+. Mitochondrial Ca2+ and Na+ 15 homeostasis, which are essential for cellular function, are controlled by exchangers and antiporters in the inner mitochondrial membrane (IMM). In the last few years, some of them have been identified, except for the mitochondrial Na+/H+ exchanger (mNHE). Here, using a rainbow of mitochondrial and nuclear genetic models, we have identified it as, specifically, the P-module of complex I (CI). In turn, its activity creates a Na+ gradient 20 across the IMM, parallel to ΔpH, which accounts for half of the ΔΨmt in coupled respiring mitochondria. We have also found that a deregulation of this mNHE function in CI, without affecting its enzymatic activity, occurs in Leber hereditary optic neuropathy (LHON), which has profound consequences in ΔΨmt and mitochondrial Ca2+ homeostasis and explains the previously unknown molecular pathogenesis of this neurodegenerative 25 disease.
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