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Title: Adaptation of the binding domain of Lactobacillus acidophilus S-layer protein as a molecular tag for affinity chromatography development
Authors: Muruaga, Emanuel J. 
Keywords: Tinh chế; protein; Sắc ký ái lực; Lactobacillus acidophilus
Issue Date: 2023
Publisher: bioRxiv
Abstract: The SLAPTAG is a novel molecular TAG derived from a protein domain present in the sequence of 22 Lactobacillus acidophilus SlpA (SlpA284-444). Proteins from different biological sources, with different 23 molecular weights or biochemical functions, can be fused in frame to the SLAPTAG and efficiently purified 24 by the specific binding to a bacterial-derived chromatographic matrix named here Bio-Matrix (BM). 25 Different binding and elution conditions were evaluated to set an optimized protocol for the SLAPTAG-26 based affinity chromatography (SAC). The binding equilibrium between SLAPTAG and BM was reached after 27 a few minutes at 4oC, being the apparent dissociation constant (KD) of 4.3 μM, a value which is similar to 28 different Kd determined for other S-layer proteins and their respective bacterial cell walls.
URI: http://dlib.hust.edu.vn/handle/HUST/23401
Link item primary: https://www.biorxiv.org/content/10.1101/2022.12.24.521862v2.full.pdf+html
Appears in Collections:OER - Kỹ thuật hóa học; Công nghệ sinh học - Thực phẩm; Công nghệ môi trường
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