Thông tin tài liệu
Title: | Bioinformatics-Guided Discovery of Biaryl-Tailored Lasso Peptides |
Authors: | Saad, Hamada |
Keywords: | tin sinh học; peptide Lasso; điều chỉnh; Biaryl |
Issue Date: | 2023 |
Publisher: | bioRxiv |
Abstract: | Lasso peptides are a class of ribosomally synthesized and post-translationally modified peptides (RiPPs) that feature an isopeptide bond and a distinct lariat fold. A growing number of secondary modifications have been described that further decorate lasso peptide scaffolds. Using genome mining, we have discovered a pair of lasso peptide biosynthetic gene clusters (BGCs) that include cytochrome P450 genes. Here, we report the structural characterization of two unique examples of (C-N) biaryl-containing lasso peptides. Nocapeptin A, from Nocardia terpenica, is tailored with Trp-Tyr crosslink while longipepetin A, from Longimycelium tulufanense, features Trp-Trp linkage. Besides the unusual bicyclic frame, longipepetin A receives an S-methylation by a new Met methyltransferase resulting in unprecedented sulfonium-bearing RiPP. Our bioinformatic survey revealed P450(s) and further maturating enzyme(s)-containing lasso BGCs awaiting future characterization. |
URI: | http://dlib.hust.edu.vn/handle/HUST/23460 |
Link item primary: | https://www.biorxiv.org/content/10.1101/2023.03.06.531328v1.full.pdf+html |
Appears in Collections: | OER - Kỹ thuật hóa học; Công nghệ sinh học - Thực phẩm; Công nghệ môi trường |
ABSTRACTS VIEWS
14
VIEWS & DOWNLOAD
4
Files in This Item:
This item is licensed under a Creative Commons License