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Title: Bioinformatics-Guided Discovery of Biaryl-Tailored Lasso Peptides
Authors: Saad, Hamada
Keywords: tin sinh học; peptide Lasso; điều chỉnh; Biaryl
Issue Date: 2023
Publisher: bioRxiv
Abstract: Lasso peptides are a class of ribosomally synthesized and post-translationally modified peptides (RiPPs) that feature an isopeptide bond and a distinct lariat fold. A growing number of secondary modifications have been described that further decorate lasso peptide scaffolds. Using genome mining, we have discovered a pair of lasso peptide biosynthetic gene clusters (BGCs) that include cytochrome P450 genes. Here, we report the structural characterization of two unique examples of (C-N) biaryl-containing lasso peptides. Nocapeptin A, from Nocardia terpenica, is tailored with Trp-Tyr crosslink while longipepetin A, from Longimycelium tulufanense, features Trp-Trp linkage. Besides the unusual bicyclic frame, longipepetin A receives an S-methylation by a new Met methyltransferase resulting in unprecedented sulfonium-bearing RiPP. Our bioinformatic survey revealed P450(s) and further maturating enzyme(s)-containing lasso BGCs awaiting future characterization.
URI: http://dlib.hust.edu.vn/handle/HUST/23460
Link item primary: https://www.biorxiv.org/content/10.1101/2023.03.06.531328v1.full.pdf+html
Appears in Collections:OER - Kỹ thuật hóa học; Công nghệ sinh học - Thực phẩm; Công nghệ môi trường
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