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DC Field | Value | Language |
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dc.contributor.author | Makarov, Dmytro | - |
dc.date.accessioned | 2023-11-21T03:09:04Z | - |
dc.date.available | 2023-11-21T03:09:04Z | - |
dc.date.issued | 2023 | - |
dc.identifier.other | OER000002663 | vi |
dc.identifier.uri | http://dlib.hust.edu.vn/handle/HUST/23527 | - |
dc.description.abstract | Tubulin detyrosination-tyrosination cycle regulates the stability of microtubules. Thus far described on α-tubulins, the tyrosination level is maintained by a single tubulin-tyrosine ligase (TTL). However, the precise dynamics and tubulin isoforms which undergo (de)tyrosination in neurons are unknown. Here, we exploit the substrate promiscuity of the TTL to introduce an O-propargyl-L-tyrosine in neuroblastoma cells and neurons. Mass spectrometry-based chemical proteomics in neuroblastoma cells using the O-propargyl-L-tyrosine probe revealed previously discussed tyrosination of TUBA4A, MAPRE1, and other non-tubulin proteins. This finding was further corroborated in differentiating neurons. Together we present the method for tubulin tyrosination profiling in living cells. Our results show that detyrosination-tyrosination is not restricted to α-tubulins with coded C-terminal tyrosine and is thus involved in fine-tuning of the tubulin and non-tubulin proteins during neuronal differentiation. | vi |
dc.description.uri | https://www.biorxiv.org/content/10.1101/2022.07.02.498566v1.full.pdf+html | vi |
dc.format | vi | |
dc.language.iso | en | vi |
dc.publisher | bioRxiv | vi |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Vietnam | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/vn/ | * |
dc.subject | Proteomics | vi |
dc.subject | hóa học | vi |
dc.subject | tyros hóa | vi |
dc.subject | protein | vi |
dc.subject | Alpha-Tubulin | vi |
dc.subject | tế bào người | vi |
dc.subject.lcc | TP248.3 | vi |
dc.title | Chemical Proteomics Reveals Protein Tyrosination Extends Beyond the Alpha-Tubulins in Human Cells | vi |
dc.type | Journal article | vi |
dc.description.note | CC BY-NC-ND 4.0 | vi |
Appears in Collections: | OER - Kỹ thuật hóa học; Công nghệ sinh học - Thực phẩm; Công nghệ môi trường |
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