Thông tin tài liệu
Title: | Three-dimensional envelope and subunit interactions of the plastid-encoded RNA polymerase from Sinapis alba |
Authors: | Ruedas, Rémi |
Keywords: | vỏ ba chiều; tiểu đơn vị; RNA polymerase; mã hóa plastid; sinh học lục lạp; protein |
Issue Date: | 2023 |
Publisher: | bioRxiv |
Abstract: | RNA polymerases (RNAPs) involved in gene transcription are found in all living organisms with degrees of complexity ranging from single polypeptide chains to multimeric enzymes. In the chloroplasts, the nuclear-encoded RNA polymerase and the plastid-encoded RNA polymerase (PEP) are both involved in the selective transcription of the plastid genome. The PEP is a prokaryotic-type multimeric RNAP found in different states depending on light stimuli and cell identity. One of its active states requires the assembly of nuclear-encoded PEP-Associated Proteins (PAPs) on the catalytic core, producing a complex of more than 900 kDa regarded as essential for chloroplast biogenesis. A purification procedure compatible with structural analysis was used to enrich the native PEP from Sinapis alba chloroplasts. |
URI: | http://dlib.hust.edu.vn/handle/HUST/23605 |
Link item primary: | https://www.biorxiv.org/content/10.1101/2022.05.04.490580v1 |
Appears in Collections: | OER - Kỹ thuật hóa học; Công nghệ sinh học - Thực phẩm; Công nghệ môi trường |
ABSTRACTS VIEWS
12
VIEWS & DOWNLOAD
13
Files in This Item:
This item is licensed under a Creative Commons License