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Title: Three-dimensional envelope and subunit interactions of the plastid-encoded RNA polymerase from Sinapis alba
Authors: Ruedas, Rémi
Keywords: vỏ ba chiều; tiểu đơn vị; RNA polymerase; mã hóa plastid; sinh học lục lạp; protein
Issue Date: 2023
Publisher: bioRxiv
Abstract: RNA polymerases (RNAPs) involved in gene transcription are found in all living organisms with degrees of complexity ranging from single polypeptide chains to multimeric enzymes. In the chloroplasts, the nuclear-encoded RNA polymerase and the plastid-encoded RNA polymerase (PEP) are both involved in the selective transcription of the plastid genome. The PEP is a prokaryotic-type multimeric RNAP found in different states depending on light stimuli and cell identity. One of its active states requires the assembly of nuclear-encoded PEP-Associated Proteins (PAPs) on the catalytic core, producing a complex of more than 900 kDa regarded as essential for chloroplast biogenesis. A purification procedure compatible with structural analysis was used to enrich the native PEP from Sinapis alba chloroplasts.
URI: http://dlib.hust.edu.vn/handle/HUST/23605
Link item primary: https://www.biorxiv.org/content/10.1101/2022.05.04.490580v1
Appears in Collections:OER - Kỹ thuật hóa học; Công nghệ sinh học - Thực phẩm; Công nghệ môi trường
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