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| Title: | HaloTag9: an engineered protein tag to improve fluorophore performance |
| Authors: | Frei, Michelle S. |
| Keywords: | HaloTag9; protein; hiệu suất; fluorophore |
| Issue Date: | 2021 |
| Publisher: | bioRxiv |
| Abstract: | HaloTag9 is an engineered variant of HaloTag7 with up to 40% higher brightness and increased fluorescence lifetime when labeled with fluorogenic rhodamines. Moreover, combining HaloTag9 with HaloTag7 and other fluorescent probes enabled live-cell multiplexing using a single fluorophore and the generation of a fluorescence lifetime-based biosensor. The increased brightness of HaloTag9 and its use in fluorescence lifetime multiplexing makes it a powerful tool for live-cell imaging |
| URI: | http://dlib.hust.edu.vn/handle/HUST/23836 |
| Link item primary: | https://www.biorxiv.org/content/10.1101/2021.04.01.438024v2.full.pdf+html |
| Appears in Collections: | OER - Kỹ thuật hóa học; Công nghệ sinh học - Thực phẩm; Công nghệ môi trường |
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