Thông tin tài liệu


Title: Pat1 increases the range of decay factors and RNA bound by the Lsm1-7 complex
Authors: Lobel, Joseph H
Gross, John D
Keywords: mRNA decay; Pat1; Lsm1; P-Bodies
Issue Date: 2020
Publisher: Biochemical Journal
Abstract: Pat1 promotes the activation and assembly of multiple proteins during mRNA decay. After deadenylation, the Pat1/Lsm1-7 complex binds to transcripts containing oligo(A) tails, which can be modified by the addition of several terminal uridine residues. Pat1 enhances Lsm1-7 binding to the 3’ end, but it is unknown how this interaction is influenced by nucleotide composition. Here we examine Pat1/Lsm1-7 binding to a series of oligoribonucleotides containing different A/U contents using recombinant purified proteins from fission yeast. We observe a positive correlation between fractional uridine content and Lsm1-7 binding affinity. Addition of Pat1 broadens RNA specificity of Lsm1-7 by enhancing binding to A-rich RNAs and increases cooperativity on all oligonucleotides tested. Consistent with increased cooperativity, Pat1 promotes multimerization of the Lsm1-7 complex, which is potentiated by RNA binding. Furthermore, the inherent ability of Pat1 to multimerize drives liquid-liquid phase separation with multivalent decapping enzyme complexes of Dcp1/Dcp2. Our results uncover how Pat1 regulates RNA binding and higher order assembly by mRNA decay factors.
Description: Tài liệu này được phát hành theo giấy phép CC-BY 4.0
URI: http://dlib.hust.edu.vn/handle/HUST/24453
Link item primary: https://www.biorxiv.org/content/10.1101/2020.04.07.029900v1
Appears in Collections:OER - Kỹ thuật hóa học; Công nghệ sinh học - Thực phẩm; Công nghệ môi trường
ABSTRACTS VIEWS

9

VIEWS & DOWNLOAD

4

Files in This Item:
Thumbnail
  • OER000000742.pdf
      Restricted Access
    • Size : 17,39 MB

    • Format : Adobe PDF



  • This item is licensed under a Creative Commons License Creative Commons