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Title: | Structure, function and antigenicity of the SARS-CoV-2 spike glycoprotein |
Authors: | Walls, Alexandra C Park, Young-Jun Tortorici, M. Alejandra |
Keywords: | Coronavirus; SARS-CoV-2; SARS-CoV; Spike glycoprotein |
Issue Date: | 2020 |
Publisher: | Biochemical Journal |
Abstract: | The recent emergence of a novel coronavirus associated with an ongoing outbreak of pneumonia (Covid-2019) resulted in infections of more than 72,000 people and claimed over 1,800 lives. Coronavirus spike (S) glycoprotein trimers promote entry into cells and are the main target of the humoral immune response. We show here that SARS-CoV-2 S mediates entry in VeroE6 cells and in BHK cells transiently transfected with human ACE2, establishing ACE2 as a functional receptor for this novel coronavirus. We further demonstrate that the receptor-binding domains of SARS-CoV-2 S and SARS-CoV S bind with similar affinities to human ACE2, which correlates with the efficient spread of SARS-CoV-2 among humans. We found that the SARS-CoV-2 S glycoprotein harbors a furin cleavage site at the boundary between the S1/S2 subunits, which is processed during biogenesis and sets this virus apart from SARS-CoV and other SARS-related CoVs. We determined a cryo-electron microscopy structure of the SARS-CoV-2 S ectodomain trimer, demonstrating spontaneous opening of the receptor-binding domain, and providing a blueprint for the design of vaccines and inhibitors of viral entry. Finally, we demonstrate that SARS-CoV S murine polyclonal sera potently inhibited SARS-CoV-2 S-mediated entry into target cells, thereby indicating that cross-neutralizing antibodies targeting conserved S epitopes can be elicited upon vaccination. |
Description: | Tài liệu này được phát hành theo giấy phép CC-BY-NC-ND 4.0 |
URI: | http://dlib.hust.edu.vn/handle/HUST/24633 |
Link item primary: | https://www.biorxiv.org/content/10.1101/2020.02.19.956581v1 |
Appears in Collections: | OER - Kỹ thuật hóa học; Công nghệ sinh học - Thực phẩm; Công nghệ môi trường |
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