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Title: PAM recognition by miniature CRISPR nucleases triggers programmable double-stranded DNA target cleavage
Authors: Karvelis, Tautvydas
Bigelyte, Greta
Young, Joshua K
Keywords: Cas9; Cas12; dsDNA
Issue Date: 2020
Publisher: Biochemical Journal
Abstract: In recent years, CRISPR-associated (Cas) nucleases have revolutionized the genome editing field. Being guided by an RNA to cleave double-stranded (ds) DNA targets near a short sequence termed a protospacer adjacent motif (PAM), Cas9 and Cas12 offer unprecedented flexibility, however, more compact versions would simplify delivery and extend application. Here, we present a collection of 10 exceptionally compact (422-603 amino acids) CRISPR-Cas nucleases that recognize and cleave dsDNA in PAM dependent manner. Categorized as class 2 type V-F they come from the Cas14 family and distantly related type V-U3 Cas proteins found in bacteria. Using biochemical methods, we demonstrate that a 5’ T- or C-rich PAM sequence triggers double stranded (ds) DNA target cleavage. Based on this discovery, we evaluated whether they can protect against invading dsDNA in E. coli and find that some but not all can. Altogether, our findings show that miniature Cas nucleases are functional CRISPR-Cas defense systems and have the potential to be harnessed as programmable nucleases for genome editing.
Description: Tài liệu này được phát hành theo giấy phép CC-BY-NC-ND 4.0
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